详情

Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies 

 Species reactivity: Human, Mouse, Rat, Goat 

 Applications: WB, IF-Cell,FC 

 MolecularWt: Predictedbandsize: 21kDa 

 Clonenumber: SZ3-0

Description: Inhealthymammaliancells, themajorityofBAXis found in thecytosol, butupon initiationof apoptoticsignaling, Bax undergoesa conformational shift. Upon induction of apoptosis, BAX becomes organelle membrane-associated, and in particular, mitochondrial membrane associated.BAX isbelieved to interactwith, and induce theopeningof themitochondrial voltage-dependent anion channel, VDAC. Alternatively, growing evidence also suggests that activated BAXand/or Bak forman oligomericpore, MAC in theMOM(mitochondrial outer membrane). This results in the releaseof cytochromecandother pro-apoptotic factors from themitochondria, often referred to asmitochondrial outer membrane permeabilization, leading to activation of caspases. Thisdefinesa direct role for BAX inmitochondrial outer membranepermeabilization. BAXactivation isstimulatedbyvarious abiotic factors, including heat, hydrogen peroxide, lowor highpH, andmitochondrial membrane remodeling. In addition, it canbecomeactivatedbybindingBCL-2, aswell asnon-BCL-2proteinssuchas p53 andBif-1. Conversely, BAXcan become inactivated by interactingwith VDAC2, Pin1, andIBRDC2