Cleaved-Caspase3p12RabbitmAbGE6325 Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies Species reactivity: Human, Pig Applications: WB, IF-Cell, IHC-P MolecularWt: Predictedbandsize: 17kDa Clonenumber: SR01-02 Description: Caspase-3 is a caspase protein that interactswith caspase-8 and caspase-9. It is encoded by theCASP3gene. CASP3orthologshavebeen identified innumerousmammals for which complete genome data are available. Unique orthologs are also present in birds, lizards, lissamphibians, and teleosts. Caspase-3 sharesmany of the typical characteristics common toall currently-knowncaspases.For example, itsactivesitecontainsacysteineresidue (Cys-163) andhistidine residue (His-121) that stabilize thepeptidebondcleavageof a proteinsequence to thecarboxy-terminal sideof anasparticacidwhen it ispart of a particular 4-aminoacidsequence. Thisspecificity allowscaspases tobe incredibly selective, witha20,000-foldpreference for asparticacidover glutamicacid.Akey featureof caspases in the cell is that they are present as zymogens, termed procaspases, which are inactive until abiochemical changecauses their activation. EachprocaspasehasanN-terminal large subunitofabout20kDa followedbyasmaller subunitofabout10kDa, calledp20andp10, respectively.